The High Throughput Screening Robotics Core represents a multi-departmental effort in combination with the Siteman Cancer Center, the Department of Radiology, the Department of Developmental Biology, and the Department of Cell Biology as well as the Howard Hughes Medical Institute. This Core serves as an outstanding on-going example of how the Molecular Imaging Center continues to stimulate interdisciplinary molecular imaging activity throughout the campus. See some of our activities here.
High Throughput Class: Bio5149: High Throughput, High Content, and You
Focal Spot Winter 2005/2006 article: "At the Core: Advances in Molecular Imaging"
Equipment
The High Throughput Screening Robotics Core is in a room that has a bio-barrier entryway with positive-pressure, HEPA filtered air circulation. Dedicated HTS Core equipment include the Beckman Coulter ORCA (optimized robot for chemical analysis) on a six foot robotic rail, Biomek FX liquid handler and rotating automated labware positioner, SAGIAN Lid Station, SAGIAN Bar Code Reader, SAGIAN Automated Tip Lift, SAGIAN microplate barcode printer, Lab-Line Microplate Shaker, Cytomat incubator and Cytomat shuttle, ELx405 Select plate reader, plate carousel, and multiDrop dispenser. Platforms incorporating the latest in molecular bioluminescence or fluorescence imaging and HTS readouts (EnVision and FluoStar Optima) are available. The HTS core also enables high content cell screens with our automated, computerized epifluorescence microscope and analysis package (GE InCell 1000). These systems enable a computer to be trained to recognize morphological and emission pattern changes at the cellular level, allowing automated screens of changes in cell biological properties.
Libraries
siRNA libraries
Compound libraries
For questions about specific applications, please contact Jayne Marasa.
Jayne Marasa
David Piwnica-Worms
McDonnell Sciences Building, Rm. 316
4565 McKinley Avenue
St. Louis, Mo 63110
Beth Cotner-Gohara
High Throughput Robotics Chemical Genetics Screening Core
4566 Scott Ave, Rm. 366
St. Louis, Mo 63110
Office: 314.362.0722
Email: bcotner@wustl.edu
Beckman Coulter
EnVision
Fluostar Optima
In Cell Analyzer 1000
