Equipment
The High Throughput Screening Robotics Core is in a room that has a bio-barrier entryway with positive-pressure, HEPA filtered air circulation. Dedicated HTS Core equipment include the Beckman Coulter ORCA (optimized robot for chemical analysis) on a 6 ft robotic rail, Biomek FX liquid handler and rotating automated labware positioner, SAGIAN Lid Station, SAGIAN Bar Code Reader, SAGIAN Automated Tip Lift, SAGIAN microplate barcode printer, Lab-Line Microplate Shaker, Cytomat incubator and Cytomat shuttle, ELx405 Select plate reader, plate carousel, and multiDrop dispenser. Platforms incorporating the latest in molecular bioluminescence or fluorescence imaging and HTS readouts (EnVision and FluoStar Optima) are available. The HTS core also enables high content cell screens with our automated, computerized epifluorescence microscope and analysis package (GE InCell 1000). These systems enable a computer to be trained to recognize morphological and emission pattern changes at the cellular level, allowing automated screens of changes in cell biological properties.
Libraries
siRNA libraries: We currently have in our freezers two libraries from Qiagen: 1) Human kinase siRNA: 646 genes; 1292 HPP Grade siRNA duplexes targeting 646 kinase and kinase-associated genes, two HPP Grade duplexes per gene. 2 nmol of each duplex, supplied in 96-well plates, 2) Human phosphatase si RNA: 222 genes; 444 HPP Grade siRNA duplexes targeting 222 phosphatase and phosphatase-related genes, two HPP Grade duplexes per gene; 2 nmol of each duplex, supplied in 96-well plates. Each plate includes non-silencing and GFP-22 siRNA controls. The human E3-ligase siRNA library is planned for purchase shortly.
Compound libraries: Libraries include the NCI Structural Diversity Set, versions 1 and 2 (1,900 compounds), Maybridge 1 (8,800 compounds), ChemDiv CombiLab and International (28,864 compounds). Plated libraries are stored in -80°C freezers. We will continue to expand our collection as libraries are planned for purchase.
